PBMCs processed from whole blood with Laminar Wash-based methods yield fewer apoptotic cells than centrifugation
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Nuclei Need Nurturing, Too
Laminar Wash™ benefits of automation and gentle cell-based sample preparation can also be extended to nuclei Analytical methods on individual cells provide an enormous amount of useful information. But not all tissue types lend themselves to preparing single cell suspensions necessary for flow cytometry or single-cell ‘omics analysis. Homogenization and dissociation of interconnected tissues […]
Towards standardizing flow cytometry sample preparation protocols without centrifugation
Now WatchingWebinar: Towards standardizing flow cytometry sample preparation protocols without centrifugation We sponsored the May 13, 2021 installment of the flowcytometryUK society webinar, hosted by renowned flow cytometrist Derek Davies, Ph.D. of the Francis Crick Institute. Curiox Applications Scientist Noma Ngwenya and Charles River Laboratories Principal Scientist III and Flow Cytometry Core Director Christoph Eberle, Ph.D. discussed how Laminar Wash™ technology supports sample prep for flow cytometry […]
A Missing Sample Prep Tool for Single-Cell Biology is Found
Gentle and semi-automated Laminar Wash™ technology removes background, debris, doublets, and dead time, extending single-cell ‘omics budgets. Single-cell ‘omics technologies are achieving a new paradigm in biology. As Eadweard Muybridge applied photography to settle the “unsupported transit” controversy surrounding the 19th century galloping horse, immunologists are utilizing single-cell transcriptomics (scRNA) to tease apart the heterogeneity […]
White Paper: Flow Cytometric HLA Crossmatch Workflow with the Curiox Laminar Wash HT2000
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Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways
READ FRONTIERS IN IMMUNOLOGY Stewart A., Ng JC-F, Wallis G, et al. Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways. Front. Immunol. 2021;12:602539. Subscribe to our newsletter Get updates about upcoming webinar technical application information and product announcements when you subscribe to the Curiox newsletter.
How to wash away barriers to therapeutic cancer vaccine development
Developing neoantigen-based cancer vaccines necessitates comprehensive cellular characterization for iterative therapeutic improvement, which Laminar Wash™ systems may facilitate at multiple steps. Vaccines are probably the second-most effective advancement in the annals of human medicine, after sanitation. Most of us recognize the value of vaccines in infectious disease. Yet, swept under the rug amongst the COVID-19 global pandemic is another form of immune-boosting injection combating a terrible […]
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
Now Watching Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries Elijah W. Chen*1, Chyan Ying Ke*2,3, Joanna Brzostek*1, Nicholas R. J. Gascoigne1, Vasily Rybakin1,41Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven * These authors […]
Developing a 43 color panel on Cytek Aurora using Laminar Wash for Sample Prep
The advent of modern flow cytometers and flow cytometric methods has accelerated researchers’ ability to enhance the resolution of samples analyzed. Within a span of 20 years, flow cytometry panels have ballooned from a handful of channels to well over 40. The theoretical limit of flow panels now lies not in the available fluorophores or […]
Washing Whole Blood Without a Centrifuge – Human and Mouse
Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage. There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new Laminar Wash™ System offers centrifuge-less blood lysis and leukocyte […]