Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
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Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
DropArray Technology allows you to easily miniaturize your multiplex assays.
Increase the number of samples you can run from a single kit.
See published data from miniaturized multiplex cytokine assays.
Useful links for multiplex immunoassays.
CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA
It is known that bead-based assays sometimes suffer from bead aggregation with plasma, blood, and other challenging biological samples which affects detectability of proteins and jeopardizes quality of data. Scientific studies have shown that DropArray may reduce bead aggregation and may better permit reading beads individually reporting the signals of target proteins as they are.
THE CURIOX
DA-BEAD PLATE
VS
TRADITIONAL PLATE
TECHNOLOGY
A) Incubation method principle in traditional plate (left) and DA-Bead Plate (right). Beads are freely floating with shaking in traditional well at 75µl volume vs. beads are fixed in a 15 µl drop in DA-Bead Plate by a magnet below the well and shaken.
(B) Washing method principle in traditional plate (left) and DA-Bead Plate (right). Magnet array with traditional plate either super packs beads at the side of a traditional well or keep beads as a monolayer resulting in aspiration of beads and/or incomplete washing with the nozzle/pipet based dispenser. Conversely, DA-Bead Plate magnet configuration maintains beads as a monolayer (right) resulting in complete washing with gentle laminar flow and minimal residual wash buffer leftover.
Multiplex bead-based assays are robust and generate highly sensitive data from a variety of samples types. However, these assays are costly and involve expensive reagents and often valuable samples. Curiox’s new DropArray™ plates allow users to analyze the same number of analytes at a fraction of the cost and sample consumption. DropArray™ plates use 5-fold smaller volumes, so the same amount of reagent can produce 5x more data points.
Peritoneal fluid cytokines show distinct delineation of endometriosis subphenotypes. Partial least squares regression (PLSR) models separated (A) ovarian endometriomas from peritoneal endometriosis, (B) ovarian endometriomas from deep infiltrating endometriosis, and (C) peritoneal endometriosis from deep infiltrating endometriosis. The principal component (PC) scores show good separation of endometriosis subphenotypes by using PF cytokines. (D–F). Corresponding PLSR coefficient analyses reveal cytokine signatures delineating the various subphenotypes. Elevated cytokines associated with a particular endometriosis subphenotype (OE, PE, or DIE) relative to its comparator appear in the same upper or lower half of the plot.
Cytokine profiles of BALB/c mice immunized with rAs37 formulated with Alhydrogel, MPL, and AddaVax. Cytokine levels were determined in supernatants of splenocytes after being re-stimulated with rAs37 (25 μg/ml) for 48 hours. Cytokine values measured in unstimulated samples were subtracted from the cytokine values from the corresponding rAs37 re-stimulated samples. Data are presented as means and individual values for each group (n = 5). *p< 0.05, **p< 0.01.
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