Curiox Laminar Wash Plates - Curiox Biosystems

LAMINAR WASH™ PLATES

Eliminate centrifugation and the experimental variables it introduces.

The wells on the Laminar Wash plate have a hydrophilic surface inside low-profile walls, including two smaller, satellite areas, and are separated by a proprietary hydrophobic surface. These uniquely engineered plates maintain each sample as a separate droplet held in place by surface tension and hydrophobic interactions with the surrounding material.

What the Wash Plates deliver:

Upgrade from centrifugation using Laminar Wash plates
Reduce hands-on time
Improve cell viability and reduce stress on cells

Enhance cell retention
Improve workflow

Technology Overview | Scientific Data | Featured Applications | Specifications | Resources

TECHNOLOGY OVERVIEW

SEE THE LAMINAR WASH HT2000 IN ACTION

Wells on the Laminar Wash plate have a hydrophilic surface inside low-profile walls, including two smaller, satellite areas, and are separated by a proprietary hydrophobic surface. These uniquely engineered plates maintain each sample as a separate droplet held in place by surface tension and hydrophobic interactions with the surrounding material.

Laminar Wash plates are used for cell processing of suspension cells for analytical assays. Each plate has 96 wells designed with two satellites to allow for dual-nozzle laminar flow processing via the HT2000 system. Each well has its own nozzles for quick and simultaneous washing of each well. Each well accomodates up to 70 ul (150 ul with an adaptor), at a maximum load of approximately 10 million cells.

“We really have a nice recovery and the Curiox (Laminar Wash technology) has been great for samples that have a very low number of cells”

Dr. Antonino Montalbano, ImmunAI

CENTRIFUGE-LESS CELL PROCESSING FOR FLOW CYTOMETRY ASSAYS

Traditional centrifuge-based preparation of cells are time consuming, labor intensive, waste expensive reagents, and stress cells. There is significant cell loss and incomplete washing resulting in problems with gating and cell segmentation.

HOW LAMINAR WASH™ WORKS

Enhanced Cell Recovery post cell sorting for lower cell numbers

The power of Curiox’s laminar cell washing technology lies in the unique design of the 96-sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.

Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.

Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.

SCIENTIFIC DATA

Laminar washing delivers better cell retention and cleaner, clearer data

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

ORDERING INFORMATION

Laminar Wash™ 16-well plate (with a regular lid)	16-DC-CL-10
*Laminar Wash™ 16-well plate (with a regular lid) Customised**	16-DC-CL-10-CP
Laminar Wash™ 96-well plate (with a regular lid)	96-DC-CL-05
*Laminar Wash™ 96-well plate (with a regular lid) Customised**	96-DC-CL-05-CP

FEATURED RESOURCES

ALL YOU NEED TO KNOW ABOUT LAMINAR WASH™ tECHNOLOGY

Walk-Away Automation of Cell Immunostaining Assays by Centrifuge-Less Laminar Wash™

Centrifuge-less Whole Blood Staining with Laminar Wash in a Biosafety Cabinet

Integrating Centrifuge Independent and Time Efficient Automated Washing in Flow Cytometry Crossmatching