Curiox Laminar Wash HT2100 System

CURIOX LAMINAR WASH HT2100 SYSTEM

Achieve higher throughputs and cleaner data with the newest member of our HT Systems

With the new Curiox Laminar Wash™ HT2100 System, you can get all the benefits of gentle laminar cell washing in a format compatible with high-throughput and automated workflows.

What the HT2100 delivers:

Better data quality and better cell recovery than centrifugation
A higher stain index for cleaner resolution of populations
Facilitate Automation with walkaway reproducibility

Reduce hands-on time, and inter-operator viability
Less cell clumping and the subsequent clogging of fluidic systems
Better biosafety through reduced aerosolization and a footprint that fits into most biosafety cabinet

TECHNOLOGY OVERVIEW

A QUICKER, SIMPLER PATH TO BETTER DATA

With the HT2100’s laminar washing technology, cells are deposited onto a 96-well laminar wash plate, where a gentle flow of buffer washes away unwanted debris, such as unbound antibody or dye. Unlike with centrifugation, very little stress is placed upon the cells, ensuring the integrity of cell surface markers. The thoroughness of the laminar washing process produces cleaner, better segregated data than centrifugation-based wash methods and the gentleness of the technique enables high cell retention and viability.

“With the Curiox Laminar Wash we retain more cells with much less data variation between samples than our centrifuge process.”

Dr Jorgen Adolfsson, Linkoping University

TRADITIONAL

WORKFLOW

LAMINAR FLOW

TECHNOLOGY

Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the HT2100 system, you can get complete washing of 96 samples in as little as 2 minutes.

SCIENTIFIC DATA

Laminar washing delivers better cell retention and cleaner, clearer data

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

Laminar wash advances with neutrophils and monocytes retention for improved differential blood count

With 100ul of human whole blood, a comparison study using a 16-color flow cytometry panel focused on T cells was conducted by Laminar Washing. Whole blood lysed with ammonium chloride buffer was first centrifuged once to reduce volume, and then aliquoted to incubate with antibodies and washed on either Laminar Wash or centrifuge in polystyrene tubes. There is clear resolution of rare T cell populations such as MAIT, gamma-delta T cells and regulatory T cells with replacement of centrifuge with Laminar Wash in one wash step. The elevated retention for neutrophils and monocytes was observed and quantified via direct acquisition onto a BD Fortessa, allowing minimal cell perturbations.

Figure 2. Human red blood cells (RBCs) lysed with (NH4Cl) and washed using HT2000 Laminar Flow technology (top) show better cell retention and tighter cell populations than RBCs lysed and washed using centrifugation on samples in tubes (bottom), whether enumerated by CD45 staining or FSC-SSC scattering.

FEATURED APPLICATIONS

The Laminar Wash system has been successfully demonstrated on various applications including immunophenotyping and CyTOF.

FLOW CYTOMETRY

Improve your sample prep workflow and gently wash cells without stress, allowing higher cell retention and less debris for acquisition and cell sorting.

SINGLE CELL SEQUENCING

Wash cells without the stress of centrifugation that may impact gene/protein expression, cell count and viability.

AUTOMATED WORKFLOWS

Easily and affordably automate labor-intensive tasks, improve data quality and standardize with Laminar Wash technology.

COVID-19 SAMPLE PREPARATION

Generate reproducible data safely while reducing aerosolization of samples and variations between users.

TECHNICAL SPECIFICATIONS

Voltage Requirements	100-240V
Power Consumption	24V/3.0A
Dimensions	310 mm H x 262mm W x 302 mm D 12.2 in H x 10.3 in W x 11.9 in D
Weight	14kg (31lbs)

ACCESSORIES AND COMPONENTS

96-well laminar wash plate (with a regular lid)	96-DC-CL-05
*96-well laminar wash plate (with a regular lid) Customised**	96-DC-CL-05
HT Buffer Exchanger 5	DC-BX-01-05
HT Buffer Exchanger 10	DC-BX-01-10
Laminar Wash 96 well Large Volume Adaptor	DC-GR-96-05
Direct Reading Grid	DC-GR02-96-05-M
Direct Reading Grid	DC-GR02-96-05-A