Curiox Laminar Wash AUTO 1000 System

THE AUTO 1000 SYSTEM

Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.

The Curiox Laminar Wash AUTO 1000 System eliminates time-consuming and labor-intensive manual pipetting and handling by automating the complete surface and intracellular staining workflow for flow and mass cytometry. You get all the benefits of laminar washing in a completely automated system.

What the AUTO 1000 delivers:

The same data quality, cell retention, cell viability, and reproducibility benefits of the HT2000 and Mini systems
An intuitive user interface that is easily programmed for those new to automation as well as automation experts
A pre-programmed, ready-to-use solution covering the entire sample prep workflow

Electronic files that can track and trace samples
The reduction in operator-to-operator variability that automation provides

Technology Overview | Scientific Data | Featured Applications | Specifications | Resources

TECHNOLOGY OVERVIEW

ACHIEVE TRULY HIGH THROUGHPUT SUSPENSION CELL SAMPLE PREPARATION

The AUTO 1000 system is a completely hands-off automated platform to dispense, fix/permeabilize, stain, wash, and transfer samples for flow and mass cytometry.

The system comes ready to use with a pre-programmed sample preparation workflow. Automation novices and experts alike can easily program a custom workflow using the intuitive user interface.

“The Laminar Wash’s performance allows us to get very tight control over populations with very tight CV’s largely due to the fact of the clear separations between populations. The maintained ability to detect rare populations is important as we detect the next round of cell therapy products.”

Arnaud Colantonio, PhD.
Associate Director of Analytical Development at AdiCet Bio,
now with Kite Pharma

HOW OUR LAMINAR FLOW TECHNOLOGY WORKS

Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the AUTO 1000, you can prepare 96 samples in just 3 minutes.

AN INNOVATIVE DESIGN DELIVERS BETTER DATA

The power of Curiox’s laminar cell washing technology lies in the unique design of the sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.

Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.

Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.

See how dye is washed away from green fluorescent polystyrene beads during 2 minutes of 7 aspiration and dispense wash cycles. At the end of the cycle, the dye is gone but the polystyrene beads remain in the spot.

SCIENTIFIC DATA

Automated laminar washing technology delivers good consistency and increased retention of low input T cell numbers from freshly thawed PBMC

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Curiox Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

Simple and straightforward reproducibility with Curiox Laminar Wash Automation

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

FEATURED APPLICATIONS

Laminar Wash technology has been successfully demonstrated on various applications including immunophenotyping and CyTOF.

FLOW CYTOMETRY

Gently wash cells without centrifugal stress, allowing higher cell retention with less debris for acquisition and cell sorting.

HLA CROSSMATCHING

Streamline sample preparation while improving reproducibility.

CELL AND GENE THERAPY

Improve reproducibility between operators, time, and locations while reducing hands on time for critical process development & CMC activities.

TUMOR MICROENVIRONMENT

Recovery of TILs can be challenging from disocciated tumor samples. Enhance cell retention, improve reproducibility and reduce debris, background, artifacts and clogging.

TECHNICAL SPECIFICATIONS

Voltage Requirements	100-240V
Power Consumption	+42V/5.0A
Dimensions	89 cm H x 71 CM W x 136 cm D 35.0 in H x 27.9 in W x 53.5 in D
Weight	120kg (264.5lbs)

ACCESSORIES AND COMPONENTS

AUTO1000 Station Standard Configuration	AUTO-1000-96-01
96-well laminar wash plate (with a regular lid)	96-DC-CL-05
HEPA Filter	AU-HE-01-01
96 well laminar wash grid (LVA)	DC-GR-96-05

FEATURED RESOURCES

ALL YOU NEED TO KNOW ABOUT THE AUTO 1000 SYSTEM

Walk-Away Automation of Cell Immunostaining Assays by Centrifuge-Less Laminar Wash™

Automated flow cytometry staining protocol

Immunophenotyping Rare Immune Cells with Laminar Wash™ AUTO System