Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
Menu
Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
Achieve cleaner samples with Laminar Wash technology
Enhance cell retention and viability with less debris
See example TILs data using flow cytometry
Publications and links to useful resources
Hence, TIL isolation and further characterization are of key importance in assessing treatment efficacy in various tumor types. However, the downstream processing from TME encounters a common problem. Typically, after tissue dissociation and isolation, TILs share their samples with high amounts of debris and dead cells in solution, contaminants needing stringent washing for removal. Instead of subjecting these precious TILs to the high mechanical stress of centrifugation that further impact retention, Laminar Wash technology offers a gentle alternative that can improve yield and data quality, particularly of rare cell types in samples containing low initial cell counts.
TRADITIONAL
CENTRIFUGATION
VS
LAMINAR FLOW
TECHNOLOGY
Tumor microenvironment and TILs isolation are difficult to prepare. Laminar Wash processing yields a clean sample while reducing hands-on time. Laminar Wash technology can:
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA
Better debris removal and cell retention:
When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting TILs cell recovery. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces the chances for accurate characterization. Here, an unchallenged mouse sample containing 1e6 TILs was prepared with Laminar Wash technology. Laminar Wash improved viable cell counts and reduced debris, compared to conventional methods. The cleaner sample preparation increases lymphocyte recovery up to 90.6%, compared to centrifuge-harsh washing of 10.5%.
Samples processed with Laminar Wash show higher viability
Splenocytes (a) and (b) and dissociated tumor cells (c) and (d) were washed with either centrifugation (manual 1 and 2) or the Laminar Wash system and viability measurements were compared. Statistical significance is reported among the manual methods vs Laminar Wash: ns = not significant, ** = P<0.01, *** = P<0.001, **** = P<0.0001. The values represent technical triplicates of the samples from an
individual naïve and an individual challenged Balb/c mouse. Manually processed samples were handled by two different analysts..
Figure adapted from Curiox Biosystems and Charles River Laboratories co-authored White Paper
