Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
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Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
Laminar Wash technology keeps samples in their native state by treating them gently.
Reduce aerosolization and enhance cell retention in your
See examples of enhanced cell retention, improved reproducibility
Publications and links to useful resources
Assessing immune responses is an indispensable means of evaluating a vaccine’s efficacy during vaccine development. When encountered with a successful vaccine antigen, our immune system is stimulated and produces a complex array of protective mechanisms.
Following a vaccine challenge, changes in frequencies of different immune cell subsets can be determined by flow cytometric immunophenotyping. Functional assays are also used to measure specific immune functions that have been induced by an effective vaccine. The immune status of immune cells can be further evaluated using Intracellular Cytokine Staining, by identifying the specific cytokines and surface markers expressed. In addition to its versatility, flow cytometry enables high flexibility in its applications.
Flow cytometry is a powerful and important technique used in vaccine development, which permits better characterization and analysis of intricate immune responses. It is unique in its ability to measure an unprecedented number of parameters, facilitating simultaneous analysis of multiple immune cell subsets.
Laminar Wash™ technology can be easily incorporated into flow cytometry sample preparation during vaccine development. Automation of cell washing using Laminar Wash™ technology reduces the time needed for sample preparation, as well as increases reproducibility between assays. Gentle cell washing by Laminar Wash technology enhances cell viability and retention, which is especially beneficial when dealing with small starting cell counts or targeting rare immune cell populations. In addition to a higher quality of data, there is an improvement in biosafety as Laminar Wash technology lowers the risk for aerosolization of infectious samples.
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TECHNOLOGY
Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.
Vaccine research and development have never been more critical for COVID-19 and other emerging diseases. The need for reliable immunological data is critical for vaccine development and clinical trials.
