DropArray Bead Plates - Curiox Biosystems

DROPARRAY BEAD PLATES

Reduce sample preparation, improve data quality and biosafety

Wall-less plate technology for miniaturizing bead-based immunoassays enabling small sample volumes (2-5𝛍l), reducing bead clumping, and saving reagents and antibodies.

Miniaturization with the DropArray-Bead plates reduces sample volume and bead consumption by 5X and decreases assay costs by >60%. Laminar flow washing is efficient and limits bead aggregat

What the DropArray Bead Plates deliver:

Sample savings
Ability to utilize existing workflows

Reduced cost of running multiplex immunoassays
Excellent retention of beads for accurate results

Technology Overview | Scientific Data | Featured Applications | Specifications | Resources

TECHNOLOGY OVERVIEW

SEE THE DROPARRAY BEAD PLATES IN ACTION

Miniaturization with the DA-Bead plates reduces sample volume and bead consumption by 5X and decreases assay costs by >60%. Laminar flow washing is efficient and limits bead aggregation.

“The DA-Bead method for analysis was comparable to, if not superior to, the conventional method in terms of consistency/precision, accuracy, sensitivity and dynamic range.”

Prof. Beaumier at Baylor and Dr. Biswas at Merck

THE CURIOX DA-BEAD PLATE

TRADITIONAL PLATE TECHNOLOGY

Washing Stations accept the Curiox DropArray™ DA-Bead and DA-HCS plates into a liquid-tight chamber formed with a clamping mechanism. After an aqueous washing buffer is automatically added, the station gently shakes the microplate to facilitate washing with minimal loss of cells. The station then tilts the microplate, using gravity to drain the excess washing buffer from the planar surface, avoiding the harsh, high-velocity liquid exchange associated with traditional washers and microplates. Buffer is added at a very low velocity, preventing cell and bead loss. In addition, there is a magnet array embedded in the plate holder for bead-based immunoassays. This helps retain magnetic beads on the microplate upon washing. Despite the low velocity, plate processing time is comparable to traditional washers because the entire plate is treated at once. After the microplate is removed from the station, the plate is ready for accepting the next set of reagents.

DROPARRAY PLATES WORK WITH EXISTING WORKFLOWS

Use your established, standard protocols with DropArray plates. The overall workflow remains the same. Available in 96-well format, the plates are offered individually or in bulk to suit your assay needs. Because they are easy to use with standard robotics, therefore there’s no need for additional capital investment. DropArray plates are compatible with magnetic beads.

HOW DA-BEAD PLATES STACK UP

	DA-Bead Plate (96-well plate)	Microplate (96-well plate)
Sample volume	10 µL*	50 µL*
Bead volume	5 µL*	25 µL**
Ease of washing	Comparable to standard microplate	N/A
No. of wells per kit	480	96
Total reagent cost of one 96-well assay	$300	$1,500
*These values may vary based on type of bead-based immunoassay.

DATA USING THE DROPARRAY LT210MX STATION

Peritoneal Fluid Cytokine Multiplex Analysis

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

Multiplex Immunoassay for a Cytokine Re-stimulation Assay

Cytokine profiles of BALB/c mice immunized with rAs37 formulated with Alhydrogel, MPL, and AddaVax. Cytokine levels were determined in supernatants of splenocytes after being re-stimulated with rAs37 (25 μg/ml) for 48 hours. Cytokine values measured in unstimulated samples were subtracted from the cytokine values from the corresponding rAs37 re-stimulated samples. Data are presented as means and individual values for each group (n = 5). *p< 0.05, **p< 0.01.

https://doi.org/10.1371/journal.pntd.0008057.g005

ACCESSORIES AND COMPONENTS

96-well DA-Bead Plate	96-CC-BD-05
384-well DA-Bead Plate	384-CC-BD-05
DropArray Bead Reading Fluid	BD-RD-01-01

FEATURED RESOURCES

ALL YOU NEED TO KNOW ABOUT THE DROPARRAY STATION

Bead-based Immunoassays with DA-Bead Plate: Milliplex Assays

Browse the latest DropArray publications

Transferring Luminex® cytokine assays to a wall-less plate technology: Validation and comparison study with plasma and cell culture supernatants.