Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
Menu
Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data
What the MINI delivers:
With the Laminar Wash technique, cells undergo a gentle wash without added stress, allowing a higher retention of cells for acquisition and cell sorting. In addition, cell surface markers are not modified and unbound antibodies are washed off efficiently, producing cleaner and better segregated data.
Each spot can process from a single cell to as many as 10 million cells without the mechanical stress and cell losses associated with centrifugation.
TRADITIONAL
WORKFLOW
VS
LAMINAR FLOW
TECHNOLOGY
After freeze-thaw and cell sorting, a limited number of peripheral blood lymphocytes (less than 500,000) is resuspended into a sorting buffer prior to performing a CiteSeq™ staining and cell hashing. As compared to three cycles of centrifugation-based washing, the percentage of cell recovered and measured to be approximately 19% as compared to Laminar Wash’s cell recovery of more than 50% in 25ul leftover volume. Data and image courtesy of ImmunAI.
Peripheral blood lymphocytes from humans were labelled with cell hashtags directly on the Laminar Wash 96 well plates and the derived sequence counts revealed less than 20% multiplets while the number of cells not stained with cell hashtag was reduced. The centrifuge-less Laminar Wash increases in singlet yield (up to 80%) which enhances more accurate identification for single cell targeting and helps higher sample multiplexing. Data and image courtesy of ImmunAI.
Laminar wash methodology yields higher cell retention and singlet distinction while enabling ease of wash protocol transfer to more complex proteogenomic reads (as seen here by up to 100 plus antibody panel). The increase in cell retention translates well to the amount of Cite-seq antibody to use and the data shows clear sample identification and clear antibody derived sequence-counts. Data and image courtesy of ImmunAI.
FLOW CYTOMETRY
|
Voltage Requirements |
100-240V |
|
Power Consumption |
24V/3.0A |
|
Dimensions |
232mm H x 275mm W x 187.5mm D 9.1in H x 10.8in W x 7.4in D |
|
Weight |
3.5kg (7.7 lbs) |
|
Laminar Wash™ 16-well plate (with a regular lid) |
96-DC-CL-05 |
|
Laminar Wash™ 16-well plate (with a regular lid) *Customised |
96-DC-CL-05-CP |
